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KMID : 0368419890320040313
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Journal of Plant Biology
1989 Volume.32 No. 4 p.313 ~ p.322
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Induction of Multi Shoots and Plant Regeneration From Protoplasts of Alfalfa(Medicago sativa L.)
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Kim, Dong Myong/金東明
Choi, Kwan Sam/Chung, Won Il/崔寬三/鄭元一
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Abstract
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A system was established for induction of multi-shoots and plant regeneration from mesophyll protoplasts of alfalfa, Medicago sativa L. cv. Vernal. Different hormonal effects were tested at each step of protoplast culture, i.e. cell division in modified Kao´s liquid medium (K566-7). calli formation on SH semi solid medium, and multi-shoot regeneration from calli on SHa and SHb solid media. Frequency of multi-shoots and plant regeneration was affected by various combinations of phytohormones in final step. The evaluation of multi-shoots induction systems via protoplast culture was discused.
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